Application
Western blot analysis of ASAH2 expression with Jurkat cell lysate (Lane 1, 2).| Product Name | ASAH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ASAH2ASAH2 is a key regulator of sphingolipid signaling metabolites. It catalyzes the degradation of sphingolipid C-16 Ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ASAH2 MGC129777 HNAC1 ASAH-2 ASAH 2 |
|---|---|
| Target | ASAH2 |
| Tissue Specificity | This antibody is specific for ASAH2. |
| Cellular Localization | The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo. |
Application
Western blot analysis of ASAH2 expression with Jurkat cell lysate (Lane 1, 2).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
