Application
Immunocytochemistry of ASC in HL60 cells with ASC antibody at 5 μg/mL.Application
Immunohistochemistry of ASC in human spleen tissue with ASC antibody at 2.5 μg/ml.Application
Immunofluorescence of ASC in human spleen tissue with ASC antibody at 20 μg/ml.Application
Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 μg /ml.WB
(E) Dose-dependent expression of pyroptosis-related proteins in untreated (control) and nicotine-treated macrophages (24 h exposure). (D) Time-dependent expression of pyroptosis-related proteins in untreated (control) and nicotine-treated macrophages (1 µM).WB
(C) The pyroptosis-related protein expression was analyzed by WB in macrophages (1 µ M nicotine, 24 h) without MT and macrophages (1 µ M nicotine, 24 h) treated with different concentrations of MT (6 h). (D) The pyroptosis-related protein expression was analyzed by WB in macrophages (1 µ M nicotine, 24 h) without MT and macrophages (1 µ M nicotine, 24 h) treated with different incubation times of 30 mM MT.WB
(B) WB analysis of the effect of caspase-1 inhibition on pyroptosis-related proteins.(D) WB analysis of the effect of caspase-1 knockdown on pyroptosis-related proteins.WB
(C) Dose-dependent expression of pyroptosis-related proteins in untreated (control) and VbP-treated macrophages (72 h exposure). (D) Time-dependent expression of pyroptosis-related proteins in untreated (control) and VbP-treated (15 μM) macrophages.WB
(G) Dose-response analysis of the expression of pyroptosis-related proteins in macrophages treated with ES (9 min exposure) after VbP treatment (15 μM, 72 h). (H) Time-dependent analysis of the expression of pyroptosis-related proteins in macrophages treated with ES (20 mv/cm) after VbP exposure (15 μM, 72 h).WB
(A) Western blot analysis of the effect of caspase-1 inhibition on pyroptosis-related proteins.WB
(D) Western blot analysis of the effect of caspase-1 knockdown on pyroptosis-related proteins.WB
(H) Western blot analysis of the effects of NAC and ES on pyroptosis-related proteins.| Product Name | ASC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.1) Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 1999;274(48):33835-8. |
| Immunogen | ASC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human ASC . The immunogen is located within the last 50 amino acids of ASC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIHC-PWB |
| ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 2.5 μg/mL..: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYCARD |
|---|---|
| Alternative Names | ASC Antibody: ASC TMS TMS1 CARD5 TMS-1 ASC Apoptosis-associated speck-like protein containing a CARD Caspase recruitment domain-containing protein 5 hASC |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunocytochemistry of ASC in HL60 cells with ASC antibody at 5 μg/mL.
Application
Immunohistochemistry of ASC in human spleen tissue with ASC antibody at 2.5 μg/ml.
Application
Immunofluorescence of ASC in human spleen tissue with ASC antibody at 20 μg/ml.
Application
Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 μg /ml.
WB
(E) Dose-dependent expression of pyroptosis-related proteins in untreated (control) and nicotine-treated macrophages (24 h exposure). (D) Time-dependent expression of pyroptosis-related proteins in untreated (control) and nicotine-treated macrophages (1 µM).
WB
(C) The pyroptosis-related protein expression was analyzed by WB in macrophages (1 µ M nicotine, 24 h) without MT and macrophages (1 µ M nicotine, 24 h) treated with different concentrations of MT (6 h). (D) The pyroptosis-related protein expression was analyzed by WB in macrophages (1 µ M nicotine, 24 h) without MT and macrophages (1 µ M nicotine, 24 h) treated with different incubation times of 30 mM MT.
WB
(B) WB analysis of the effect of caspase-1 inhibition on pyroptosis-related proteins.(D) WB analysis of the effect of caspase-1 knockdown on pyroptosis-related proteins.
WB
(C) Dose-dependent expression of pyroptosis-related proteins in untreated (control) and VbP-treated macrophages (72 h exposure). (D) Time-dependent expression of pyroptosis-related proteins in untreated (control) and VbP-treated (15 μM) macrophages.
WB
(G) Dose-response analysis of the expression of pyroptosis-related proteins in macrophages treated with ES (9 min exposure) after VbP treatment (15 μM, 72 h). (H) Time-dependent analysis of the expression of pyroptosis-related proteins in macrophages treated with ES (20 mv/cm) after VbP exposure (15 μM, 72 h).
WB
(A) Western blot analysis of the effect of caspase-1 inhibition on pyroptosis-related proteins.
WB
(D) Western blot analysis of the effect of caspase-1 knockdown on pyroptosis-related proteins.
WB
(H) Western blot analysis of the effects of NAC and ES on pyroptosis-related proteins.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
|---|---|
| Application Notes | ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 2.5 μg/mL..: |
| Form | Liquijavascript:void(0);d |
|---|---|
| Storage Instructions | ASC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ASC Antibody is supplied in PBS containing 0.02% sodium azide. |
