Application
Immunofluorescence of ATP11B in K562 cells with ATP11B antibody at 20 μg/mL.Application
Immunocytochemistry of ATP11B in K562 cells with ATP11B antibody at 10 μg/mL.Application
Western blot analysis of ATP11B in K562 cell tissue lysate with ATP11B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ATP11B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP11B Antibody: ATPIF, ATPIR, ATPIF, KIAA0956, Probable phospholipid-transporting ATPase IF, ATPase IR |
| Product description | ATP11B Antibody: ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.1) Mansharamani M, Hewetson A, and Chilton BS. Cloning and characterization of an atypical Type IV P-type ATPase that binds to the RING motif of RUSH transcription factors. J. Biol. Chem.2001; 276:3641-9. |
| Immunogen | ATP11B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11B. The immunogen is located within amino acids 290 - 340 of ATP11B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ATP11B antibody can be used for detection of ATP11B by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP11B |
|---|---|
| Alternative Names | ATP11B Antibody: ATPIF ATPIR ATPIF KIAA0956 Probable phospholipid-transporting ATPase IF ATPase IR |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunofluorescence of ATP11B in K562 cells with ATP11B antibody at 20 μg/mL.
Application
Immunocytochemistry of ATP11B in K562 cells with ATP11B antibody at 10 μg/mL.
Application
Western blot analysis of ATP11B in K562 cell tissue lysate with ATP11B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ATP11B antibody can be used for detection of ATP11B by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATP11B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATP11B Antibody is supplied in PBS containing 0.02% sodium azide. |
