P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu (2+) or Cd (2+). Another subfamily transports non-heavy metal ions, such as H (+), Na (+), K (+), or Ca (+). A third subfamily transports amphipaths, such as phosphatidylserine.P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu (2+) or Cd (2+). Another subfamily transports non-heavy metal ions, such as H (+), Na (+), K (+), or Ca (+). A third subfamily transports amphipaths, such as phosphatidylserine.1) Muzny, D.M., (2006) Nature 440 (7088), 1194-1198.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP11B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ATP11B antibody can be used for detection of ATP11B by ELISA at 1:312500. ATP11B antibody can be used for detection of ATP11B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
ATP11B antibody can be used for detection of ATP11B by ELISA at 1:312500. ATP11B antibody can be used for detection of ATP11B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP11B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.