Application
Western Blot at 1:2000 dilution Lane 1: human fetal heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.| Product Name | ATP5G1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP synthase F(0) complex subunit C1, mitochondrial, ATP synthase lipid-binding protein, ATP synthase proteolipid P1, ATP synthase proton-transporting mitochondrial F(0) complex subunit C1, ATPase protein 9, ATPase subunit c, ATP5G1 |
| Product description | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.1) Dyer M.R.,et al.Biochem. J. 293:51-64(1993). |
| Immunogen | This ATP5G1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the Central region of human ATP5G1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP5G1 |
|---|---|
| Alternative Names | ATP synthase F(0) complex subunit C1 mitochondrial ATP synthase lipid-binding protein ATP synthase proteolipid P1 ATP synthase proton-transporting mitochondrial F(0) complex subunit C1 ATPase protein 9 ATPase subunit c ATP5G1 |
| Molecular Weight(MW) | 14 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: human fetal heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
