Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] diluted at 1:500.Application
PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSAT1 protein stained by PSAT1 antibody [GT1353] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] diluted at 1:1000.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] diluted at 1:1000.| Product Name | PSAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1353] to PSAT1 (phosphoserine aminotransferase 1)The protein encoded by this gene is likely a phosphoserine aminotransferase, based on similarity to proteins in mouse, rabbit, and Drosophila. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT1353 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, BovineRat (95%), Xenopus laevis (82%), Rabbit (94%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPIP antibody PSA antibody PSAT antibody PSAT1 antibody [GT1353] antibody PSAT1 antibody phosphoserine aminotransferase 1 antibody |
|---|---|
| Target | PSAT1 |
| Molecular Weight(MW) | 40 kDa (note) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] diluted at 1:500.
Application
PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSAT1 protein stained by PSAT1 antibody [GT1353] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] diluted at 1:1000.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] diluted at 1:1000.| Positive Control | 293T , A549 , H1299 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
