Application
ApplicationApplication
staining PSCA in human breast carcinoma by Immunohistochemistry (FFPE-sections).Application
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody| Product Name | PSCA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PSCAPSCA (Prostate Stem Cell antigen) is a cell surface antigen, which is overexpressed in ~40% of primary prostate cancers and in as many as 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas. Antibody directed against PSCA inhibits tumorigenesis, slows tumor growth, prolongs survival and prevents metastasis in a preclinical zenograft model indicating that PSCA may have utility as a prognostic marker and/or therapeutic target in prostate cancer. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (extracellular). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| For IHC-P: Use at a dilution of 1:75 for 10 min at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 8000 PRO232 O43653 602470 PSCA |
|---|---|
| Target | PSCA |
| Cellular Localization | Cell membrane and cytoplasmic |
Application
Application
Application
staining PSCA in human breast carcinoma by Immunohistochemistry (FFPE-sections).
Application
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody| Positive Control | Bladder carcinoma and Prostate carcinoma |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:75 for 10 min at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
