Application
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibodyApplication
Application| Product Name | PSCA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to PSCA ( Prostate Stem Cell Antigen )This antibody reacts with a ~14 kD protein. Prostate Stem Cell Antigen (PSCA) is a cell surface antigen which is overexpressed in ~ 40% of primary prostate cancers and in nearly 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas. Antibody directed against PSCA inhibits tumorigenesis, slows tumor growth, prolongs survival and prevents metastasis in a preclinical zenograft model. |
| Immunogen | A synthetic peptide derived from extracellular region of human PSCA protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 8000 PRO232 O43653 602470 PSCA |
|---|---|
| Target | PSCA |
| Cellular Localization | Cell membrane, cytoplasmic |
Application
Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody
Application
Application| Positive Control | Bladder carcinoma, prostate carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
