Application
ICC of PSD95 in cultures of dissociated hippocampal neurons. Cultures were grown and labeled with antibodies against the indicated proteins. A neuron double stained for PSD95.Application
PSD95 visualized using tested on HaCat cells.Application
Western blot analysis of PSD95 in rat membrane using a 1:1000 dilution of| Product Name | PSD95 antibody [6G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6G6] to PSD95Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase(MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an Nterminal variable segment followed by three aminoterminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7). Selected References 1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25. 2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277(5331): 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5. |
| Immunogen | Recombinant rat PSD-95 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 6G6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| We recommend a working dilution of 1:250 (WB), 1:1000 (IHC), and 1:100 (ICC/IF). | |
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | SAP90, DLG4 |
|---|---|
| Target | PSD95 |
| Molecular Weight(MW) | 100kDa (note) |
| Cellular Localization | High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. |
Application
ICC of PSD95 in cultures of dissociated hippocampal neurons. Cultures were grown and labeled with antibodies against the indicated proteins. A neuron double stained for PSD95.
Application
PSD95 visualized using tested on HaCat cells.
Application
Western blot analysis of PSD95 in rat membrane using a 1:1000 dilution of| Application Notes | We recommend a working dilution of 1:250 (WB), 1:1000 (IHC), and 1:100 (ICC/IF). |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS pH7.4, 50% glycerol |
