Application
Immunocytochemistry of BAFF in HL60 cells with BAFF antibody at 1 μg/mL.Application
Immunofluorescence of BAFF in human spleen tissue with BAFF antibody at 20 μg/ml.Application
Western blot analysis of BAFF in human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with BAFF antibody at 1 μg/mL.| Product Name | BAFF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.1) Moore PA , Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999;285:260-3 |
| Immunogen | BAFF antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF. The immunogen is located within the last 50 amino acids of BAFF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| BAFF antibody can be used for detection of BAFF by Western blot a 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF13B |
|---|---|
| Alternative Names | BAFF Antibody: DTL BAFF BLYS CD257 TALL1 THANK ZTNF4 TALL-1 TNFSF20 UNQ401/PRO738 Tumor necrosis factor ligand superfamily member 13B B lymphocyte stimulator |
| Molecular Weight(MW) | 31 kDa |
| Sequence Similarities | 3) Cat. No. 17-009 - HL-60 Cell Slide |
Application
Immunocytochemistry of BAFF in HL60 cells with BAFF antibody at 1 μg/mL.
Application
Immunofluorescence of BAFF in human spleen tissue with BAFF antibody at 20 μg/ml.
Application
Western blot analysis of BAFF in human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with BAFF antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
|---|---|
| Application Notes | BAFF antibody can be used for detection of BAFF by Western blot a 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAFF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAFF Antibody is supplied in PBS containing 0.02% sodium azide. |
