| Product Name | PSE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [N9C1] to PSE1Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq] |
| Immunogen | Proximal sequence element (PSE1) of the human U1 small nuclear RNA extracted from human placenta. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | N9C1 |
| Host Species | Mouse |
| Tested Applications | EMSAIPWB |
| Western blot and electrophoretic mobility (gel shift) assays for identifying PSE1 or Ku. This antibody may be used to immuno-deplete Ku protein from protein mixtures prior to specific DNA affinity chromatography. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | FLJ43041, IMB3, KPNB3, MGC2068, PSE1, DKFZp686O1576, RANBP5 |
|---|---|
| Target | PSE1 |
| Tissue Specificity | Recognizes the large alpha subunit (~80-90 kDa) of placental PSE1 and human Ku in solution and when bound to DNA. |
| Application Notes | Western blot and electrophoretic mobility (gel shift) assays for identifying PSE1 or Ku. This antibody may be used to immuno-deplete Ku protein from protein mixtures prior to specific DNA affinity chromatography. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
