Application
Immunohistochemistry of BAP29 in human heart tissue with BAP29 antibody at 10 μg/mL.Application
Immunofluorescence of BAP29 in Human Heart cells with BAP29 antibody at 20 μg/mL.Application
Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.| Product Name | BAP29 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAP29 Antibody: Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins. Bap29 possesses multiple isoforms.1) Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J.1994; 13:3793-800. |
| Immunogen | BAP29 antibody was raised against a 17 amino acid synthetic peptide from near the center of human Bap29 Bap29 antibody is specific for isoform a of Bap29. The immunogen is located within amino acids 130 - 180 of BAP29. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bap29 antibody can be used for the detection of Bap29 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAP29 |
|---|---|
| Alternative Names | BAP29 Antibody: BAP29 BAP29 B-cell receptor-associated protein 29 BCR-associated protein 29 |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Entrez Gene | 55973 |
|---|---|
| Protein Accession | NP_001008405 |
Application
Immunohistochemistry of BAP29 in human heart tissue with BAP29 antibody at 10 μg/mL.
Application
Immunofluorescence of BAP29 in Human Heart cells with BAP29 antibody at 20 μg/mL.
Application
Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | Bap29 antibody can be used for the detection of Bap29 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAP29 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAP29 Antibody is supplied in PBS containing 0.02% sodium azide. |
