BAP29 Antibody: Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins. Bap29 possesses multiple isoforms.1) Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J.1994; 13:3793-800.
Immunogen
BAP29 antibody was raised against a 17 amino acid synthetic peptide from near the center of human Bap29 Bap29 antibody is specific for isoform a of Bap29. The immunogen is located within amino acids 230 - 280 of BAP29.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAWB
Bap29 antibody can be used for the detection of Bap29 by Western blot at 1 - 2 μg/mL.
Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 1 and (B) 2 μg/mL.
Positive Control
1) Cat. No. 1301 - Human Heart Tissue Lysate
Application Notes
Bap29 antibody can be used for the detection of Bap29 by Western blot at 1 - 2 μg/mL.
Additional Information
Form
Liquid
Storage Instructions
BAP29 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
BAP29 Antibody is supplied in PBS containing 0.02% sodium azide.