Application
Immunohistochemical staining of human brain tissue using BAP3 antibody at 2.5 μg/mL.Application
Immunofluorescence of BAP3 in human brain tissue with BAP3 antibody at 20 μg/mL.Application
Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at 2 μg/mL.| Product Name | BAP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAP3 Antibody: BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.1) Shiratsuchi T, Oda K, Nishimori H, et al. Cloning and characterization of BAP (BAI-associated protein 3), a C2 domain-containing protein that interacts with BAI1. Biochem. Biophys. Res. Comm.1998; 251:158-65. |
| Immunogen | BAP3 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human BAP3. The immunogen is located within the first 50 amino acids of BAP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| BAP3 antibody can be used for the detection of BAP3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAIAP3 |
|---|---|
| Alternative Names | BAP3 Antibody: BAP3 KIAA0734 BAI1-associated protein 3 BAP3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemical staining of human brain tissue using BAP3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of BAP3 in human brain tissue with BAP3 antibody at 20 μg/mL.
Application
Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | BAP3 antibody can be used for the detection of BAP3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
