Application
Immunohistochemical staining of human brain tissue using BAP3 antibody at 5 μg/mL.Application
Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BAP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAP3 Antibody: BAP3 was initially identified through interaction in a yeast two-hybrid system with the brain-specific angiogenesis inhibitor 1, a p53-target gene that encodes a seven-span transmembrane protein member of the secretin receptor family. BAP3 is predominantly expressed in the brain and possess high homology with Munc13 and synaptotagmin, suggesting that BAP3 may play a role in regulating neurotransmitter release. Recent experiments have shown that BAP3 is induced in certain tumors such as desmoplastic small round cell tumor. Ectopic expression of BAP3 in tumor cells dramatically enhances growth in low serum conditions and colony formation in soft agar, suggesting that the regulated exocytotic pathway may play a role in cancer cell proliferation.1) Shiratsuchi T, Oda K, Nishimori H, et al. Cloning and characterization of BAP (BAI-associated protein 3), a C2 domain-containing protein that interacts with BAI1. Biochem. Biophys. Res. Comm.1998; 251:158-65. |
| Immunogen | BAP3 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human BAP3. The immunogen is located within amino acids 60 - 110 of BAP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| BAP3 antibody can be used for the detection of BAP3 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAIAP3 |
|---|---|
| Alternative Names | BAP3 Antibody: BAP3 KIAA0734 BAI1-associated protein 3 BAP3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | BAP3 is known to have two isoforms; this BAP3 antibody will recognize only isoform 2. Lower molecular weight bands may represent cleavage or degradation products. |
| Entrez Gene | 8938 |
|---|---|
| Protein Accession | NP_001273393 |
Application
Immunohistochemical staining of human brain tissue using BAP3 antibody at 5 μg/mL.
Application
Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | BAP3 antibody can be used for the detection of BAP3 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
