Application
BCAT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | BCAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.1) Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010) |
| Immunogen | This BCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-107 amino acids from the Central region of human BCAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAT1 |
|---|---|
| Alternative Names | Branched-chain-amino-acid aminotransferase cytosolic BCAT(c) Protein ECA39 BCAT1 BCT1 ECA39 |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
BCAT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
