Application
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.Application
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL.Application
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.| Product Name | Bcl-G Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.1) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607. |
| Immunogen | Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G. The immunogen is located within the first 50 amino acids of Bcl-G. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2L14 |
|---|---|
| Alternative Names | Bcl-G Antibody: BCLG BCLG Apoptosis facilitator Bcl-2-like protein 14 Apoptosis regulator Bcl-G Bcl2-L-14 |
| Sequence Similarities | 2) Cat. No. 1416 - Mouse Testis Tissue Lysate |
| Tissue Specificity | Although antibody should react with both isoforms, only the Bcl-GS protein has been observed |
Application
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.
Application
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL.
Application
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1215 - U937 Cell Lysate |
|---|---|
| Application Notes | Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide. |
