WB
Western blot analysis of Bcl G/BCL2L14 on different lysates with Rabbit anti-Bcl G/BCL2L14 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: K-562 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: RAW264.7 cell lysate, Lane 6: C6 cell lysate, Lane 7: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Bcl G/BCL2L14 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL2L14 |
|---|---|
| Gene Synonyms | BCLG |
| Gene Full Name | BCL2 like 14 |
| Gene Summary | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of this gene has been shown to induce apoptosis in cells. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported for this gene. [provided by RefSeq, May 2009] |
| Alternative Names | Apoptosis facilitator Bcl 2 like protein 14 antibody Apoptosis regulator BCL G antibody Bcl2 L 14 antibody BCL2 like 14 (apoptosis facilitator) antibody BCL2 like 14 antibody BCL2L14 antibody BCLG antibody testicular tissue protein Li 26 antibody |
| Molecular Weight(MW) | 37kDa(Observed band size:45kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Bcl G/BCL2L14 on different lysates with Rabbit anti-Bcl G/BCL2L14 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: K-562 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: RAW264.7 cell lysate, Lane 6: C6 cell lysate, Lane 7: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
