Application
Western blot analysis of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 μg/mL.Application
Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 μg/mL.| Product Name | Beclin-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. |
| Immunogen | Beclin-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beclin-1. The immunogen is located within the last 50 amino acids of Beclin-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BECN1 |
|---|---|
| Alternative Names | Beclin-1 Antibody: ATG6 VPS30 beclin1 GT197 Beclin-1 Coiled-coil myosin-like BCL2-interacting protein |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
Application
Western blot analysis of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 μg/mL.
Application
Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Beclin-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
