Application
Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.Application
Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.| Product Name | Beta III Tubulin Antibody [AA10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)1) Lee MK, Rebhun LI, Frankfurter A (1990) Posttranslational modification of class III beta tubulin. Proc Natl Acad Sci USA 87(18): 7195-7199. |
| Immunogen | Synthetic peptide corresponding to beta III tubulin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Species Reactivity | Mammalian species |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | Tubb3 |
|---|---|
| Alternative Names | Neuron-specific class III beta-tubulin |
| Molecular Weight(MW) | 55 |
| Tissue Specificity | Specific for the ~55kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1. |
Application
Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.
Application
Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.| Form | Liquid |
|---|---|
| Storage Instructions | Beta III Tubulin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
