Application
Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 10 μg/mL.Application
Immunofluorescence of Bfl-1 in Mouse Kidney tissue with Bfl-1 antibody at 20 μg/mL.Application
Western blot analysis of Bfl-1 in (A) human kidney and (B) human lung tissue lysate with Bfl-1 antibody at 1 μg/mL.| Product Name | Bfl-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2A1 |
|---|---|
| Alternative Names | Bfl-1 Antibody: GRS BFL1 ACC-1 ACC-2 HBPA1 BCL2L5 GRS Bcl-2-related protein A1 Bcl-2-like protein 5 Bcl2-L-5 |
| Molecular Weight(MW) | Predicted: 19 kDa Observed: 20 kDa |
| Sequence Similarities | 3) Cat. No. 1302 - Human Lung Tissue Lysate |
| Tissue Specificity | At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform. |
Application
Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 10 μg/mL.
Application
Immunofluorescence of Bfl-1 in Mouse Kidney tissue with Bfl-1 antibody at 20 μg/mL.
Application
Western blot analysis of Bfl-1 in (A) human kidney and (B) human lung tissue lysate with Bfl-1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bfl-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
