Application
Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 2 μg/mL.Application
Immunofluorescence of Bfl-1 in Mouse Kidney tissue with Bfl-1 antibody at 20 ug/mL.Application
Western blot analysis of Bfl-1 in human kidney tissue lysate with Bfl-1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bfl-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Bfl-1. The immunogen is located within the first 50 amino acids of Bfl-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2A1 |
|---|---|
| Alternative Names | Bfl-1 Antibody: GRS BFL1 ACC-1 ACC-2 HBPA1 BCL2L5 GRS Bcl-2-related protein A1 Bcl-2-like protein 5 Bcl2-L-5 |
| Molecular Weight(MW) | Predicted: 18, 19 kDa Observed: 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (87%) |
| Tissue Specificity | At least two isoforms of Bfl-1 are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 2 μg/mL.
Application
Immunofluorescence of Bfl-1 in Mouse Kidney tissue with Bfl-1 antibody at 20 ug/mL.
Application
Western blot analysis of Bfl-1 in human kidney tissue lysate with Bfl-1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bfl-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
