Application
Immunocytochemistry of BIM EL inK562 cells with BIM EL antibody at 2.5 μg/ml.Application
Immunofluorescence of BIM EL in K562 cells with BIM EL antibody at 2.5 μg/ml.Application
Western blot analysis of Bim in K562 cell lysate with Bim EL antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bim EL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bim EL Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim, also known as Bcl-2-like protein 11, is a pro-apoptotic member of this family and interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Multiple isoforms of Bim are known to exist, with Bim EL being the longest isoform (1,2).1) O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395. |
| Immunogen | Bim EL antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bim. The immunogen is located within amino acids 20 - 70 of Bim EL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Bim EL antibody can be used for detection of Bim EL by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2L11 |
|---|---|
| Alternative Names | Bim EL Antibody: BAM BIM BOD Bcl-2-like protein 11 Bcl2-interacting mediator of cell death Bcl2-L-11 |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 24 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | Bim EL antibody is human and mouse reactive. Multiple isoforms of Bim are known to exist; this antibody only detects the Bim EL isoform. |
Application
Immunocytochemistry of BIM EL inK562 cells with BIM EL antibody at 2.5 μg/ml.
Application
Immunofluorescence of BIM EL in K562 cells with BIM EL antibody at 2.5 μg/ml.
Application
Western blot analysis of Bim in K562 cell lysate with Bim EL antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Bim EL antibody can be used for detection of Bim EL by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bim EL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Bim EL Antibody is supplied in PBS containing 0.02% sodium azide. |
