Application
Western blot analysis of extracts from COS7 cells (Lane 2), using BL-CAM (Phospho-Tyr807) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | BL-CAM (phospho Tyr807) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.1) Wilson G.L., J. Exp. Med. 173-137-146(1991). |
| Immunogen | BL-CAM (phospho Tyr807) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 807 (G-D-Y (p)-E-N) derived from Human BL-CAM. |
| Modification | p-Tyr807 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD22 |
|---|---|
| Alternative Names | Siglec-2 B-cell receptor CD22 BL-CAM Leu-14 B-lymphocyte cell adhesion molecule |
| Molecular Weight(MW) | 95 kDa |
| Tissue Specificity | The antibody detects endogenous levels of BL-CAM only when phosphorylated at tyrosine 807. |
| Entrez Gene | 933 |
|---|---|
| Protein Accession | NP_001762.2 |
Application
Western blot analysis of extracts from COS7 cells (Lane 2), using BL-CAM (Phospho-Tyr807) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
