Application
Immunohistochemistry of GCPII in human Medulla oblongata by GCP-04 monoclonal antibody . Mag. 40x; positive astrocytes in white matter.Application
Immunohistochemistry of GCPII in human prostate by GCP-04 monoclonal antibody . Mag. 400x; positive epithelium of the prostate glands.Application
Immunohistochemistry of GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.Application
Immunostaining of a fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody . Lanes 1, 2, 3 represent 800, 400 and 200 pg of the protein.| Product Name | PSMA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GCP-04] to PSMAGlutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer disease, Huntington disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM? than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.Entrez Gene (human): 11p11.2 |
| Immunogen | Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GCP-04 |
| Host Species | Mouse |
| Tested Applications | IDICC/IFIHCIHC-PWB |
| WB: Use at 1 µg/mL. Positive Control: LNCaP cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE Sample buffer. Note: Both reducing and non-reducing conditions: |
|
| Species Reactivity | HumanPigRat |
| Concentration | 1mg/ml |
| Gene Synonyms | FGCP FOLH GCP2 GCPII NAALAD1 NAALAdase PSM PSMA mGCP |
|---|---|
| Target | GCPII |
| Tissue Specificity | The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein. |
Application
Immunohistochemistry of GCPII in human Medulla oblongata by GCP-04 monoclonal antibody . Mag. 40x; positive astrocytes in white matter.
Application
Immunohistochemistry of GCPII in human prostate by GCP-04 monoclonal antibody . Mag. 400x; positive epithelium of the prostate glands.
Application
Immunohistochemistry of GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.
Application
Immunostaining of a fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody . Lanes 1, 2, 3 represent 800, 400 and 200 pg of the protein.| Application Notes | WB: Use at 1 µg/mL. Positive Control: LNCaP cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE Sample buffer. Note: Both reducing and non-reducing conditions: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
