Application
Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | BTK (phospho Tyr223) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGMX1, ATK, kinase Btk, BPK |
| Product description | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.1) Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676. |
| Immunogen | BTK (phospho Tyr223) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y (p)-D-Y) derived from Human BTK. |
| Modification | p-Tyr223 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BTK |
|---|---|
| Alternative Names | AGMX1 ATK kinase Btk BPK |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | The antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223. |
| Entrez Gene | 695 |
|---|---|
| Protein Accession | NP_000052.1 |
Application
Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
