Application
Immunofluorescense analysis of Jurkat cells using BTK monoclonal antibody with FITC - IgG (top). Immunohistochemical analysis of paraffin - embedded human lymph follicle tissue showing cytoplasmic and membrane location using BTK antibody with DAB (bottom).Application
Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.| Product Name | BTK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGMX1, ATK, BPK, Agammaglobulinemia tyrosine kinase, AT, XLA, IMD1, AGMX1, PSCTK1 |
| Product description | Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.1) Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240. |
| Immunogen | Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | BTK |
|---|---|
| Alternative Names | AGMX1 ATK BPK Agammaglobulinemia tyrosine kinase AT XLA IMD1 AGMX1 PSCTK1 |
Application
Immunofluorescense analysis of Jurkat cells using BTK monoclonal antibody with FITC - IgG (top). Immunohistochemical analysis of paraffin - embedded human lymph follicle tissue showing cytoplasmic and membrane location using BTK antibody with DAB (bottom).
Application
Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BTK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
