Application
Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 μg/mL.Application
Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/mL.Application
Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 μg/ml.Application
Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 μg/mL.| Product Name | BTK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BTK Antibody: AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase |
| Product description | BTK Antibody: Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.1) Vetrie D, Vorechovsky I, Sideras P, et al. The gene involved in X-linked aγglobulinaemia is a member of the src family of tyrosine kinases. Nature 1993; 361:226-33. |
| Immunogen | BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK. The immunogen is located within amino acids 20 - 70 of BTK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BTK |
|---|---|
| Alternative Names | BTK Antibody: AT ATK BPK XLA IMD1 AGMX1 PSCTK1 Tyrosine-protein kinase BTK Agammaglobulinemia tyrosine kinase |
| Molecular Weight(MW) | Predicted: 76 kDa Observed: 79 kDa |
| Sequence Similarities | 2) Cat. No. 1224 - Daudi Cell Lysate |
| Tissue Specificity | Multiple isoforms of BTK are known to exist. |
Application
Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 μg/mL.
Application
Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/mL.
Application
Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 μg/ml.
Application
Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1215 - U937 Cell Lysate |
|---|---|
| Application Notes | BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BTK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BTK Antibody is supplied in PBS containing 0.02% sodium azide. |
