Application
Flow cytometric analysis of Hela cells, using anti-PSMD10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-PSMD10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMD10 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)| Product Name | PSMD10 antibody [3F6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3F6] to PSMD10Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and TP53/p53. Overexpression is leading to phosphorylation of RB1 and proteosomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to TP53/p53 and the mono- and polyubiquitination of TP53/p53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. |
| Immunogen | Full length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F6 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | dJ889N15.2, PSMD10, p28 |
|---|---|
| Target | PSMD10 |
| Tissue Specificity | is specific for human PSMD10 (NP_002805). |
Application
Flow cytometric analysis of Hela cells, using anti-PSMD10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-PSMD10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMD10 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
