Application
The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system| Product Name | PSMD11 antibody [AT1F4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT1F4] to PSMD11PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins. |
| Immunogen | Recombinant human PSMD11 (1-422aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT1F4 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:250 ~ 1000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | S9, PSMD11, macropain) 26S subunit non-ATPase 11, macropain) 26S subunit nonATPase 11, p44.5, Rpn6, macropain) 26S subunit nonATPase11, proteasome (prosome, MGC3844 |
|---|---|
| Target | PSMD11 |
| Light Chain Type | kappa |
Application
The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system| Application Notes | Western Blot: Use at 1:250 ~ 1000. Optimal working concentrations should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
