Application
Western blot analysis of extracts of various cell lines, using PSME3 antibody.Application
Immunofluorescence analysis of HeLa cell using PSME3 antibody. Blue: DAPI for nuclear staining.| Product Name | PSME3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSME3The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulat |
| Immunogen | Recombinant protein of human PSME3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PA28-gamma Ki PA28G proteasome (prosome macropain) activator subunit 3 (PA28 gamma Ki) REG-GAMMA PA28gamma |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using PSME3 antibody.
Application
Immunofluorescence analysis of HeLa cell using PSME3 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
