Application
Immunohistochemical analysis of paraffin - embedded human brain glioma tissue showing nuclear localization using calcyclin antibody with DAB staining.Application
Western blot analysis using calcyclin monoclonal antibody against truncated calcyclin recombinant protein| Product Name | Calcyclin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Calcyclin encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.1) Bean C.et.al J Mol Biol. 2005 Jun 3;349(2):349-66. |
| Immunogen | GSTs Resin purified truncated recombinant calcyclin expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:Dilution 1:200 - 1:1,000 IHC(P):Dilution 1:100 - 1:500 IHC(F):Dilution 1:100 - 1:500 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CACYBP |
|---|---|
| Alternative Names | S100A6BP SIP PNAS-107 S100A6-binding protein CacyBP GIG5 S100A6BP RP1-102G20.6 |
Application
Immunohistochemical analysis of paraffin - embedded human brain glioma tissue showing nuclear localization using calcyclin antibody with DAB staining.
Application
Western blot analysis using calcyclin monoclonal antibody against truncated calcyclin recombinant protein| Application Notes | Western Blot:Dilution 1:200 - 1:1,000 IHC(P):Dilution 1:100 - 1:500 IHC(F):Dilution 1:100 - 1:500 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Calcyclin monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
