Application
Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 ug/mL in either the (A) absence or (B) presence of blocking peptide.| Product Name | PTEN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTENThis gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Immunogen | PTEN antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PTEN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| WB: 1 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 10q23del CWS1 phosphatase and tensin homolog PTEN1 TEP1 BZS MMAC1 DEC GLM2 MHAM |
|---|---|
| Target | PTEN |
Application
Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 ug/mL in either the (A) absence or (B) presence of blocking peptide.| Application Notes | WB: 1 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
