Application
Western blot analysis of PTEN in P815 cell lysate with PTEN (NT) antibody at (A) 1 and (B) 2 ug/mL.Application
Immunofluorescence of PTEN in P815 cells with PTEN antibody at 2 ug/mL.| Product Name | PTEN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTENThis gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Immunogen | PTEN antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PTEN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| WB: 1 - 2 ug/ml. IF: 2 ug/ml: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TEP1 GLM2 DEC MMAC1 BZS MHAM 10q23del CWS1 phosphatase and tensin homolog PTEN1 |
|---|---|
| Target | PTEN |
Application
Western blot analysis of PTEN in P815 cell lysate with PTEN (NT) antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunofluorescence of PTEN in P815 cells with PTEN antibody at 2 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. IF: 2 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
