Application
Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 μg/mL.Application
Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Caspase-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1 |
| Product description | Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-1. The immunogen is located within the last 50 amino acids of Caspase-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP1 |
|---|---|
| Alternative Names | Caspase-1 Antibody: ICE P45 IL1BC IL1BCE Caspase-1 Interleukin-1 beta convertase CASP-1 |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | Depending on cell lines or tissues used, other cleavage products may be observed. |
Application
Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 μg/mL.
Application
Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
