Application
Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 μg/mL.Application
Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 μg/mL.Application
Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Caspase-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1 |
| Product description | Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. CCell 2004; 117:561-74. |
| Immunogen | Caspase-1 antibody was raised against a 15 amino acid synthetic peptide from near the middle of human Caspase-1. The immunogen is located within amino acids 200 - 250 of Caspase-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP1 |
|---|---|
| Alternative Names | Caspase-1 Antibody: ICE P45 IL1BC IL1BCE Caspase-1 Interleukin-1 beta convertase CASP-1 |
| Sequence Similarities | 4) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Depending on cell lines or tissues used, other cleavage products may be observed. |
Application
Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 μg/mL.
Application
Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 μg/mL.
Application
Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
