Application
Western blot analysis of caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1 μg/mL.| Product Name | Caspase-10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-10 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.1) Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1β-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 1997;272:6578-6583 |
| Immunogen | Caspase-10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Caspase-10. The immunogen is located within amino acids 240 - 290 of Caspase-10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Caspase-10 antibody can be used for detection of caspase-10 by Western blot 1 μg/mL. A 59 kDa band can be detected.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP10 |
|---|---|
| Alternative Names | Caspase-10 Antibody: MCH4 Caspase-10 Apoptotic protease Mch-4 CASP-10 |
Application
Western blot analysis of caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1 μg/mL.| Application Notes | Caspase-10 antibody can be used for detection of caspase-10 by Western blot 1 μg/mL. A 59 kDa band can be detected.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-10 Antibody is supplied in PBS containing 0.02% sodium azide. |
