Application
WB analysis of (1) HeLa, (2) MCF7, (3) 293T and (4) A431 lysates (10 μg per lane) using PTEN antibody [EPR9941] at a dilution of 1:1000.Application
IHC-P analysis of human colon tissue using PTEN antibody [EPR9941] at a dilution of 1:50.| Product Name | PTEN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR9941] to PTENThis gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant full length Human PTEN protein (UniProt: P60484). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR9941 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CWS1 TEP1 DEC BZS GLM2 MMAC1 10q23del PTEN1 phosphatase and tensin homolog MHAM |
|---|---|
| Target | PTEN |
Application
WB analysis of (1) HeLa, (2) MCF7, (3) 293T and (4) A431 lysates (10 μg per lane) using PTEN antibody [EPR9941] at a dilution of 1:1000.
Application
IHC-P analysis of human colon tissue using PTEN antibody [EPR9941] at a dilution of 1:50.| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at -20°C. |
| Storage Buffer | Preservative: 0.01% Sodium azide. Constituents: 50% Glycerol, 0.05% BSA. |
