Application
Immunofluorescent staining of HT29 cells using anti-PTPRE mouse monoclonal antibody .Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.Application
HEK293T cells transfected with either pCMV6-ENTRY PTPREApplication
Anti-PTPRE mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PTPRE| Product Name | PTPRE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2C11] to PTPREIsoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function. Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake. Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+). |
| Immunogen | Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2C11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp313F1310 HPTPE RPTPEPSILON PTPRE PTPE R-PTP-EPSILON R PTP EPSILON |
|---|---|
| Target | PTPRE |
| Tissue Specificity | is specific for human PTPRE (NP_006495). |
| Cellular Localization | Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane. |
Application
Immunofluorescent staining of HT29 cells using anti-PTPRE mouse monoclonal antibody .
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Application
HEK293T cells transfected with either pCMV6-ENTRY PTPRE
Application
Anti-PTPRE mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PTPRE| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
