Application
Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.| Product Name | CD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD4mut |
| Product description | The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximately 58 kDa contains four external domains (D1 to D4) that show homology to members of the immunoglobulin (Ig) superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.1) M Benkirane J Virol. 1995 November; 69(11): 6898¨C6903. |
| Immunogen | Ni-NTA purified recombinant human CD4 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot, IHC and ELISA. Dilution: Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD4 |
|---|---|
| Alternative Names | CD4mut |
| Entrez Gene | 920 |
|---|---|
| Protein Accession | NP_000607.1 |
Application
Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.| Positive Control | 1) Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate |
|---|---|
| Application Notes | Western Blot, IHC and ELISA. Dilution: Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CD4 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
