Application
Flow cytometric analysis of HepG2 cells using CEACAM Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CEACAM antibody in HepG2 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CEACAM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CEACAM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, CD66a, CEACAM1, BGP, BGP1 |
| Product description | CEACAM is a member of the carcinoembryonic antigen (CEA) family, which belongs to the immunoglobulin superfamily. The protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.1) Gambichler,T., Am. J. Clin. Pathol. 131 (6), 782-787 (2009) |
| Immunogen | This CEACAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-77 amino acids from the N-terminal region of human CEACAM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CEACAM1 |
|---|---|
| Alternative Names | Carcinoembryonic antigen-related cell adhesion molecule 1 Biliary glycoprotein 1 BGP-1 CD66a CEACAM1 BGP BGP1 |
| Molecular Weight(MW) | 58 kDa |
Application
Flow cytometric analysis of HepG2 cells using CEACAM Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CEACAM antibody in HepG2 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CEACAM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
