Application
CES2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: human liver lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: human liver lysates Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488Application
Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Product Name | CES2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance.1) Holmes, R.S., et al. Mamm. Genome 21 (9-10), 427-441 (2010)Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Howard, T.D., et al. Environ. Health Perspect. 118(10):1395-1399(2010) Hatfield, M.J., et al. Br. J. Pharmacol. 160(8):1916-1928(2010) Holmes, R.S., et al. Genetica 138(7):695-708(2010) |
| Immunogen | This CES2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 340-369 amino acids from the Central region of human CES2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CES2 |
|---|---|
| Alternative Names | Cocaine esterase Carboxylesterase 2 CE-2 hCE-2 Methylumbelliferyl-acetate deacetylase 2 CES2 ICE |
| Molecular Weight(MW) | 62 kDa |
Application
CES2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: human liver lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: human liver lysates Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488
Application
Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
