Application
Immunohistochemistry of CFTR in human small intestine tissue with CFTR antibody at 5 μg/mL.Application
Immunofluorescence of CFTR in human small intestine tissue with CFTR antibody at 20 μg/mL.Application
Western blot analysis of CFTR in human small intestine tissue lysate with CFTR antibody at 1 μg/ml.| Product Name | CFTR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR |
| Product description | The cystic fibrosis transmembrane conductance regulator (CFTR) protein is a member of the ATP-binding cassette (ABC) transporter superfamily, and a member of the MRP subfamily that is involved in multi-drug resistance (1,2). CFTR functions as a chloride channel and controls the regulation of other transport pathways (3). Mutations in this gene are associated with the autosomal recessive disorder cystic fibrosis, the most common, fatal, inherited disease of caucasian populations (1).1) Kerem B, Rommens JM, Buchanan JA, et al. Identification of the cystic fibrosis gene: genetic analysis. Science 1989; 245:1073-80. |
| Immunogen | CFTR antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CFTR. The immunogen is located within amino acids 1290 - 1340 of CFTR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CFTR antibody can be used for detection of CFTR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFTR |
|---|---|
| Alternative Names | CFTR Antibody: CF MRP7 ABC35 ABCC7 CFTR/MRP TNR-CFTR dJ760C5.1 Cystic fibrosis transmembrane conductance regulator ATP-binding cassette sub-family C member 7 CFTR |
| Molecular Weight(MW) | Predicted: 163 kDa Observed: 160 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | CFTR antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of CFTR in human small intestine tissue with CFTR antibody at 5 μg/mL.
Application
Immunofluorescence of CFTR in human small intestine tissue with CFTR antibody at 20 μg/mL.
Application
Western blot analysis of CFTR in human small intestine tissue lysate with CFTR antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | CFTR antibody can be used for detection of CFTR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CFTR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CFTR Antibody is supplied in PBS containing 0.02% sodium azide. |
