WB
WB analysis using CFTR Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CFTR antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human lung using CFTR Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human lung tissue slide using CFTR antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | CFTR Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CFTR protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:5000-1:50000 IHC:1:250-1:1000 |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CFTR |
|---|---|
| Gene Synonyms | CF MRP7 ABC35 ABCC7 CFTR/MRP TNR-CFTR dJ760C5.1 |
| Gene Full Name | CF transmembrane conductance regulator |
| Gene Summary | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Alternative Names | Channel conductance-controlling ATPase CFTR/MRP cAMP-dependent chloride channel ATP-binding cassette sub-family C member 7 ABCC7 |
| Molecular Weight(MW) | 168 kDa(Observed band size:150 kDa) |
| Cellular Localization | Cell membrane,Endoplasmic reticulum,Endosome,Membrane,Nucleus. |
WB
WB analysis using CFTR Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CFTR antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human lung using CFTR Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human lung tissue slide using CFTR antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:5000-1:50000 IHC:1:250-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
