CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX (9)C-CX (9)C- motif in the C terminus.CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX (9)C-CX (9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).1) Terziyska, N., (2007) FEBS Lett. 581 (6), 1098-1102.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHCHD4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CHCHD4 antibody can be used for detection of CHCHD4 by ELISA at 1:62500. CHCHD4 antibody can be used for detection of CHCHD4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
CHCHD4 antibody can be used for detection of CHCHD4 by ELISA at 1:62500. CHCHD4 antibody can be used for detection of CHCHD4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHCHD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.