Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
MIA40 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | MIA40 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).1) Daithankar, V.N., et al. Biochemistry 48(22):4828-4837(2009) |
| Immunogen | This MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the C-terminal region of human MIA40. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHCHD4 |
|---|---|
| Alternative Names | Mitochondrial intermembrane space import and assembly protein 40 Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 CHCHD4 MIA40 |
| Molecular Weight(MW) | 16 kDa |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
MIA40 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
