The CHRNA3 subunit is expressed in the soma of the majority of pyramidal cells, with the most alpha 3 immunoreactivity observed in CA2-4 and entorhinal cortex and relatively less in CA1 and subicular pyramidal cell soma.1) Boorman, J.P., et al., (2003) J. Biol. Chem. 278 (45), 44033-44040.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNA3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CHRNA3 antibody can be used for detection of CHRNA3 by ELISA at 1:312500. CHRNA3 antibody can be used for detection of CHRNA3 by western blot at 0.1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
CHRNA3 antibody can be used for detection of CHRNA3 by ELISA at 1:312500. CHRNA3 antibody can be used for detection of CHRNA3 by western blot at 0.1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHRNA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.