Application
CHRNA3 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of CHRNA3 Antibody in CEM cell line lysates (35ug/lane)| Product Name | CHRNA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuronal acetylcholine receptor subunit alpha-3, CHRNA3, NACHRA3 |
| Product description | CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.1) Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010) |
| Immunogen | This CHRNA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-52 amino acids from the N-terminal region of human CHRNA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHRNA3 |
|---|---|
| Alternative Names | Neuronal acetylcholine receptor subunit alpha-3 CHRNA3 NACHRA3 |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
CHRNA3 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of CHRNA3 Antibody in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
