The CHRNA3 subunit is expressed in the soma of the majority of pyramidal cells, with the most alpha 3 immunoreactivity observed in CA2-4 and entorhinal cortex and relatively less in CA1 and subicular pyramidal cell soma.1) Arredondo, J., et al., (2005) Am. J. Pathol. 166 (2), 597-613.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNA3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CHRNA3 antibody can be used for detection of CHRNA3 by ELISA at 1:1562500. CHRNA3 antibody can be used for detection of CHRNA3 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
CHRNA3 antibody can be used for detection of CHRNA3 by ELISA at 1:1562500. CHRNA3 antibody can be used for detection of CHRNA3 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHRNA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt