Application
Immunohistochemical analysis of paraffin - embedded human glioma tissue showing membrane location using CIB1 antibody with DAB staining.Application
Western blot analysis using CIB1 monoclonal antibody against truncated CIB1 recombinant protein and A431 cell lysate.| Product Name | CIB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIB, CIBP, KIP1, PRKDCIP, SIP2-28 |
| Product description | CIB1(also designated calcium and integrin binding 1 or calmyrin),with 191-amino acid protein(about 21 kDa), belongs to the calcium-binding protein family.CIB1 is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining.CIB1 is an EF-hand-containing protein that binds multiple effector proteins, including the platelet alpha(IIb)beta(3) integrin and several serine/threonine kinases and potentially modulates their function.CIB1 regulates platelet aggregation in hemostasis through a specific interaction with the alpha(IIb) cytoplasmic domain of platelet integrin alpha(IIb)beta(3). CIB1 is also ubiquitously expressed activating and inhibiting protein ligand of the InsP3R.1) Holly R. Gentry,Alex U. Singer, Laurie Betts. J. Biol. Chem., Mar 2005; 280: 8407 - 8415. |
| Immunogen | Ni-NTA purified truncated recombinant CIB1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CIB1 |
|---|---|
| Alternative Names | CIB CIBP KIP1 PRKDCIP SIP2-28 |
| Entrez Gene | 10519 |
|---|---|
| Protein Accession | NP_001264693.1 |
Application
Immunohistochemical analysis of paraffin - embedded human glioma tissue showing membrane location using CIB1 antibody with DAB staining.
Application
Western blot analysis using CIB1 monoclonal antibody against truncated CIB1 recombinant protein and A431 cell lysate.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIB1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
